Stationary Phase Retention
From TheLiquidPhase
(Redirected from Sf)
Stationary Phase Retention (Sf) is the ratio between the Stationary Phase volume and the total Column (system) volume (eq. 1). The larger the Sf, the larger the column (system) capacity available for the separation, hence, the better the resolution. In general, higher rotation speeds of the column (system) and lower flow rates of the Mobile Phase improve Sf. Vmp is equal to the volume of stationary phase eluted during the equilibrium (see below)